Al-Khalifa, M. S., Diab, F. M., & Khalil, G. M. (2007). Integration: Selective Replication Compentent: No - needs helper virus (CMV, adenovirus, herpesvirus, vaccinia) RCV Testing: If helper virus is adenovirus, presence of RCV after heat inactivation (56C for 15min) Recombination: Possible. During the acute phase, typical symptoms are arthritis, itching rash, fatigue, mild fever, headache, and muscle pain that usually occur within the first 2 days of illness Footnote 9. CHARACTERISTICS: Of the genus Alphavirus, and a member of the Togaviridae family. Kurkela, S., Helve, T., Vaheri, A., & Vapalahti, O. Users are responsible for ensuring they are compliant with all relevant acts, regulations, guidelines, and standards. Experiments using this virus need to be approved by the administrative panel on biosafety of your institution. For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, [email protected], (406) 994-6733 or Safety and Risk Management (406-994- 2711). Thrushes are the main amplifying hosts, but also the fieldfare and redwing. Laboratory Biosafety Guidelines (3rd ed.). Emergency Management and Business Continuity, Student Health Plan Administration and Enrollment. Smit, J. M., Bittman, R., & Wilschut, J. Handling of sharps (needles, scalpels, microtome blades, broken glass. LABORATORY-ACQUIRED INFECTIONS: None reported to date. Arthritis and arthralgia three years after Sindbis virus infection: Clinical follow-up of a cohort of 49 patients. Griffin, D. E. (2001). Alphaviral vectors based on Semliki Forest virus and Sindbis virus infect many host cell types, causing rapid and high‐level transgene expression. Clinical and laboratory manifestations of sindbis virus infection: Prospective study, Finland, 2002-2003. Newly discovered hazards are frequent and this information may not be completely up to date. Other changes in the bloodfeeding behavior of Aedes aegypti after dissemination of Sindbis virus were observed after exposure to repellents Decontamination and Disinfection. Risk assessments and mitigation measures are dependent on: 1. Generation of high-titer virus stocks is rapid (less than two days) and typically requires biosafety level 1 or 2 containment. PHYSICAL INACTIVATION: The virus is sensitive to temperatures above 58°C Footnote 21. Virions are spherical to pleomorphic (70 nm in diameter), consisting of an envelope and nucleocapsid. Krauss, H., Schiefer, H. G., Weber, A., Slenczka, W., Appel, M., von Graevenitz, A., Enders, B., Zahner, H., & Isenberg, H. D. (2003). (2002). As the lowest of the four, biosafety level 1 applies to laboratory settings in which personnel work with low-risk microbes that pose little to no threat of infection in healthy adults. skin lesions, rash), Musculoskeletal symptoms (i.e. Virions are spherical to pleomorphic (70 nm in diameter), consisting of an envelope and nucleocapsid. For assistance, contact MSU's Biosafety Officer: Ryan Bartlett, [email protected], (406) 994-6733 or Safety and Risk Management (406-994- 2711). Semliki Forest virus (SFV) and Sindbis virus (SIN) are two, positive-strand RNA viruses of the alphavirus genus. DRUG SUSCEPTIBILITY: No specific treatment Footnote 2 ; however, dioxane-based antiviral agents for treatment of Sindbis virus disease are under investigation Footnote 18 . Biological Safety: Principles and Practices (4th ed., pp. Since wild-type Sindbis virus have a pathogenicity (it causes rash-arthritis syndrome in human), Sindbis virus is classified as a Biosafety Level-2 agent by the NIH Recombinant DNA Advisory Committee. Turell, M. J., Morrill, J. C., Rossi, C. A., Gad, A. M., Cope, S. E., Clements, T. L., Arthur, R. R., Wasieloski, L. P., Dohm, D. J., Nash, D., Hassan, M. M., Hassan, A. N., Morsy, Z. S., & Presley, S. M. (2002). Infection with Sindbis virus begins with a sudden onset of fever, headache, and joint pain. die normaal geen ziekte bij de mens veroorzaken. Prolonged arthritis associated with Sindbis-related (Pogosta) virus infection. Journal of Medical Virology, 23(3), 297-301. mosquitoes in Egypt. Laine, M., Luukkainen, R., & Toivanen, A. RESERVOIR: Wild birds are the natural reservoir (probably asymptomatic) Footnote 7. (2000). These requirements restrict the ability of public health laboratories to conduct PRNTs. Lelie, P. N., Reesink, H. W., & Lucas, C. J. PATHOGENICITY/TOXICITY: Sindbis fever in humans is a self-limiting, mild, febrile disease with vesicular exanthema and arthralgia; however, clinically unapparent infections are common. Medlock, J. M., Snow, K. R., & Leach, S. (2007). The virus was first isolated in 1952 in Cairo, Egypt. 8 Sindbis virus infections were mild; 2 of 3 fatal cases in- ... tained for 2 MIDV strains under Biosafety Level 3 con-ditions. mosquitoes in Egypt. Additional precautions should be considered with work involving animals or large scale activities Footnote 27. Saudi Medical Journal, 28(12), 1864-1867. Biological safety cabinets and other physical containment devices should be used whenever procedures with high potential for generating aerosols are conducted. Journal of Medical Entomology, 39(1), 248-250. Generation of high-titer virus stocks is rapid (less than two days) and typically requires biosafety level 1 or 2 containment. SYNONYM OR CROSS REFERENCE: Epidemic polyarthritis and rash, Sindbis virus disease, SIN, SINV, Ockelbo disease or August-September disease in Sweden Footnote 3, Pogosta disease in Finland, and Karelian fever in Russia1-6. FIRST AID/TREATMENT: Treatment is symptomatic. 3. Here, we exploit a biosafety level 2 (BSL-2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors directed against the papain-like protease (PLpro) of severe acute respiratory syndrome coronavirus (SARS-CoV), a biosafety level 3 (BSL-3) pathogen. Laine, M., Luukkainen, R., Jalava, J., Ilonen, J., Kuusistö, P., & Toivanen, A. Work with Sindbis virus (SINV) and many other old-world alphaviruses not associated with severe disease in humans can be conducted at Biosafety Level -2 (BSL-2). Personnel Protection Required (minimum requirement): Sindbis virus (SINV) a single-stranded, positive, enveloped RNA virus belonging to the western equine encephalomyelitis complex of the genus Alphavirus in the family Togaviridae. The first human cases were described in Uganda in 1961, South Africa in 1963, and Australia in 1967. Sindbis virus infection in resident birds, migratory birds, and humans, finland. (2008). Favero, M. S., & Arduino, M. J. Since wild-type Sindbis virus have a pathogenicity (it causes rash-arthritis syndrome in human), Sindbis virus is classified as a Biosafety Level-2 agent by the NIH Recombinant DNA Advisory Committee. Er zijn geen speciale veiligheidsmaatregelen nodig anders dan een goed geoutilleerd microbiologisch laboratorium.Gewone laboratoriumhygiëne is voldoende. The rash is distributed diffusely over the trunk and limbs and affects the palms and soles. Biological Safety Manuals and Other Documents, Sindbis Virus Biological Agent Reference Sheet (BARS), Fire Protection Impairment and Fire Watch, Infectious Disease Preparedness for Event Planners, Mobile Vending and Food Truck Operations Requirements, Other Resources and Procedures for Safety, Hazard Assessment and Signage Program (HASP), Dry Ice, Formaldehyde, Lithium Batteries, GMO's. Biosafety Levels for Viral Vectors│ www.ehs.washington.edu │September 10, 2020 Page 1 of 1. pp. Exposure Procedures Washington D.C.: Pan American Health Organization. Journal of Virological Methods, 134(1-2), 171-175. Gould, E. A., Higgs, S., Buckley, A., & Gritsun, T. S. (2006). Lundström, J. O., Lindström, K. M., Olsen, B., Dufva, R., & Krakower, D. S. (2001). Live virus is used in the PRNT procedure, which currently requires biosafety level 3 containment facilities and select agent security in the case of EEEV. Call 911 from a campus phone or 607-255-1111 from a mobile phone. SOURCES/SPECIMENS: The virus can be isolated from infected mosquitoes Footnote 26, skin lesions and whole blood or serum samples of febrile patients Footnote 3Footnote 10. An IgM capture ELISA is available for the demonstration of virus-specific IgM antibody and is mostly used for the diagnosis. Possible ecology and epidemiology of medically important mosquito-borne arboviruses in Great Britain. The non-segmented genome consists of a single molecule of linear positive-sense single-stranded RNA Footnote 3Footnote 7Footnote 8. SURVIVAL OUTSIDE HOST: Sindbis virus can infect and survive in cell culture at low temperature and low pH Footnote 24Footnote 25. Sindbis Virus Biological Agent Reference Sheet (BARS) Print this page Disclaimer : Risk group, biosafety level, and all other precautions noted here are subject to change after a risk assessment by EHS. The genome of MIDV isolate SAE25/2011 (GenBank accession no. Emerging zoonoses and vector-borne infections affecting humans in Europe. (2006). IgM increases during the acute phase of the disease and then tends to decrease slowly for 3 to 4 years, independent of persistent symptoms Footnote 10. Heymann, D. L. (2004). Splash/droplet-creating activities (shaking incubators, liquid culturing. Potential arbovirus emergence and implications for the United Kingdom. REGULATORY INFORMATION: The import, transport, and use of pathogens in Canada is regulated under many regulatory bodies, including the Public Health Agency of Canada, Health Canada, Canadian Food Inspection Agency, Environment Canada, and Transport Canada. The use of needles, syringes, and other sharp objects should be strictly limited. Sindbis virus (SINV) is an enveloped RNA virus of the genus Alphavirus in the virus family Togaviridae. Consisting of an envelope and nucleocapsid biological activities as inhibitors of Sindbis virus, mosquitoes with disseminated infection took and!, M. 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